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KMID : 1134820000290040712
Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 4 p.712 ~ p.718
Physiological Characteristics of Anticoagulant Fractions from Eugenia caryophyllata
Lee Jong-im

Lee Hyun-Sun
Jun Woo-Jin
Yoo Kwang-Won
Shin Dong-hoon
Hong Bum-Shik
Cho Hong-Yon
Yang Han-Chul
Abstract
The alkali extraction of anticoagulant from a clove revealed 3-to 6-fold more effective than hot-water extraction. The highest anticoagulant activity was found with 0.1 N NaOH at 70¡É. The anticoagulant fractions from a clove, EC-2B and EC-2C were separated by alkali extraction, ethanol precipitation, cetavlon treatment, and ultrafiltration. The anticoagulant activities of these two fractions were, respectively, 6.57 and 8.63 times higher than those extracted with hot-water. As of the sensory evaluation, boiled pork added with EC-2B fraction revealed similar sensory acceptability to raw clove material, while EC-2C fraction had low sensory acceptability due to a mild chemical odor. Antibacterial characteristics against pathogenic microorganism of both fractions were confirmed in the control strain. The inhibitory effect of growth by EC-2B was noticed above 0.016% in S. aureus. Also, EC-2C showed the inhibitory effect at 0.004% in both E. coli and S. aureus control strains.
KEYWORD
Eugenia caryophyllata, anticoagulant, sensory evaluation, antivacterial
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